5-Formylcytosine anticorps

N° du produit ABIN2668977

Cet anticorps Lapin Polyclonal détecte spécifiquement 5-Formylcytosine dans DB, ICC, IF et WB. Il a été cité dans 1 publication.

Aperçu rapide pour 5-Formylcytosine anticorps (ABIN2668977)

Antigène: 5-Formylcytosine

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Détail du produit

Fonction: 5-Formylcytosine (5-fC) antibody (pAb)

Purification: Protein A Chromatography

Immunogène: This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.

Isotype: IgG

Information d'application

Indications d'application: DB: 0.5 - 2 μg/mL dilution

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Référence

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." dans: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Détail du antigène

Antigène: 5-Formylcytosine

Classe de substances: Chemical

Sujet: DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.