5-Methylcytosine (5MeC) anticorps

N° du produit ABIN2668982

Cité dans 10 publications. Cet anticorps anti-5-Methylcytosine Monoclonal Souris (Clone 33D3) (ABIN2668982) détecte spécifiquement 5-Methylcytosine dans FACS, IF, MeDIP, DB, ICC, ELISA et IHC.

Aperçu rapide pour 5-Methylcytosine (5MeC) anticorps (ABIN2668982)

Antigène: 5-Methylcytosine (5MeC)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Methylated DNA Immunoprecipitation (MeDIP), Dot Blot (DB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (IHC)

Clone: 33D3

Détail du produit

Fonction: 5-Methylcytosine (5-mC) antibody (mAb) (Clone 33D3)

Purification: Protein A Chromatography

Immunogène: Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.

Isotype: IgG

Information d'application

Indications d'application: Validated Applications: MeDIP: 1 μg per IP IHC (FFPE): 1:1000 ELISA: 1:10,000 dilution Published Applications: MeDIP ICC/IF Flow Cytometry IHC DB

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.4).

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Référence

Borghesan, Fusilli, Rappa, Panebianco, Rizzo, Oben, Mazzoccoli, Faulkes, Pata, Agodi, Rezaee, Minogue, Warren, Peterson, Sedivy, Douet, Buschbeck, Cappello, Mazza, Pazienza, Vinciguerra: "DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression." dans: Cancer research, Vol. 76, Issue 3, pp. 594-606, (2016) (PubMed).

McPherson, Fullston, Kang, Sandeman, Corbett, Owens, Lane: "Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers." dans: Scientific reports, Vol. 6, pp. 27010, (2016) (PubMed).

Banh, Iorio, Marcotte, Xu, Cojocari, Rahman, Pawling, Zhang, Sinha, Rose, Isasa, Zhang, Wu, Virtanen, Hitomi, Habu, Sidhu, Koizumi, Wilkins, Kislinger, Gygi, Schofield, Dennis, Wouters, Neel: "PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia." dans: Nature cell biology, Vol. 18, Issue 7, pp. 803-813, (2016) (PubMed).

Harrison, Cornett, Goldfarb, DaRosa, Li, Yan, Dickson, Guo, Cantu, Kaustov, Brown, Arrowsmith, Erie, Major, Klevit, Krajewski, Kuhlman, Strahl, Rothbart: "Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1." dans: eLife, Vol. 5, (2016) (PubMed).

Yang, Ma, Li, Zhang, Wan, Du, Li, Yang, Lu: "Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression." dans: Oncology letters, Vol. 12, Issue 4, pp. 2789-2795, (2016) (PubMed).

Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." dans: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).

Xu, Wang, Long, Zhang, Wei, Xing, Gu, Xie: "Stress-mediated p38 activation promotes somatic cell reprogramming." dans: Cell research, Vol. 23, Issue 1, pp. 131-41, (2013) (PubMed).

Colquitt, Allen, Barnea, Lomvardas: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).

Bocker, Tuorto, Raddatz, Musch, Yang, Xu, Lyko, Breiling: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." dans: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).

Habib, Fares, Bourgeois, Bella, Bernardino, Hernandez-Blazquez, de Capoa, Niveleau: "DNA global hypomethylation in EBV-transformed interphase nuclei." dans: Experimental cell research, Vol. 249, Issue 1, pp. 46-53, (1999) (PubMed).

Détail du antigène

Antigène: 5-Methylcytosine (5MeC)

Autre désignation: 5-Methylcytosine

Classe de substances: Chemical

Sujet: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.