O-GlcNAc anticorps
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- Antigène
- O-GlcNAc
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Reactivité
- Chemical
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Inconjugué
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purification
- IgM purification
- Immunogène
- This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and threonine.
- Clone
- CTD110-6
- Isotype
- IgM
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 1 μg/μL
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Stock
- -20 °C
- Stockage commentaire
- Antibodies in solution can be stored at -20 °C for 2 years.
- Date de péremption
- 6 months
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-
Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc." dans: Nature chemical biology, Vol. 6, Issue 5, pp. 338-43, (2010) (PubMed).
: "Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine." dans: Analytical biochemistry, Vol. 293, Issue 2, pp. 169-77, (2001) (PubMed).
: "
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Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc." dans: Nature chemical biology, Vol. 6, Issue 5, pp. 338-43, (2010) (PubMed).
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- Antigène
- O-GlcNAc
- Classe de substances
- Viral Protein
- Sujet
- O-GlcNAc (b-O-linked N-acetylglucosamine) is a ubiquitous and dynamic post-translational modification present on numerous cytoplasmic and nuclear proteins and is thought to play a regulatory role in numerous cellular processes including homeostatic mechanisms and gene expression. OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residues whereas OGA / O-GlcNAcase (O- linked N-acetylglucosamine (GlcNAc)-ase) is a glycosidase that removes O-GlcNAc. O-GlcNAc modifications have been described to directly regulate the activities of a variety of transcription factors including Sp1, estrogen receptors, STAT5, NF-κB, p53, YY1, Elf-1, c-Myc, Rb, PDX-1, CREB, fork-head, and others. In addition, RNA polymerase II and the basal transcription factors are also extensively modified by O-GlcNAc. O-GlcNAc modification has been reported to suppress or enhance transcription, depending on the promoter and other associated proteins.
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