Biotin anticorps (PE)

N° du produit ABIN2669897

N° du produit (Fournisseur): 700-108-098

Cet anticorps Chèvre Polyclonal détecte spécifiquement Biotin dans WB, ELISA et FACS. Il présente une réactivité envers .

Aperçu rapide pour Biotin anticorps (PE) (ABIN2669897)

Antigène: Biotin

Fragment: F(ab')2 fragment

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la PE

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-Biotin anticorps (PE)

N° du produit (Fournisseur): 700-108-098

Fournisseur: Rockland

Fonction: F(Ab')2 Biotin Antibody Phycoerythrin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum and Biotinylated IgG.

Attributs du produit: Synonyms: Goat F(ab')2 Anti-Biotin Phycoerythrin Conjugated Antibody, Goat F(ab')2 Anti Biotin PE Conjugated Antibody, Goat Fab2 Anti-Biotin Antibody Phycoerythrin Conjugation

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogène:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotype: IgG

Information d'application

Indications d'application:

Flow Cytometry Dilution: 1 μg/1,000,000 cells

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:100-1:250

ELISA Dilution: 1:100-1:250

Other: Flow Cytometry 1 μg/1,000,000 cells

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Date de péremption: 12 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers Background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.