CD180 anticorps (Biotin)

N° du produit ABIN2689038

L’anticorps Rat Monoclonal anti-CD180 a été validé pour FACS. Il convient pour détecter CD180 dans des échantillons de Souris. Il y a 7+ publications disponibles.

Aperçu rapide pour CD180 anticorps (Biotin) (ABIN2689038)

Antigène: CD180 (CD180 Molecule (CD180))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD180 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: RP-14

Détail du produit anti-CD180 anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: The RP/14 antibody reacts with CD180, the "radioprotective" 105 kDa (RP105) antigen expressed on mature B lymphocytes. Like other members of the Toll-like Receptor (TLR) family, the extracellular region of CD180 contains tandem repeats of a leucine-rich motif flanked by regions containing conserved cysteines. These characteristic structural components of TLR may mediate protein-protein interactions and regulation of signal transduction involved in innate immune responses. Furthermore, CD180 associates with the secretory protein MD-1 on the cell surface. Cross-linking of cell-surface CD180 with the RP/14 antibody induces activation signals in B lymphocytes. Despite the expression of CD180 on lymphocytes of all strains tested (BALB/c, BALB/c.xid, C3H, C57BL/6, CBA/N, and 129/Sv), some strains (BALB/c.xid, C57BL/6.xid, CBA/N, 129/Sv, and 129/Ola) display deficient responses to that stimulatory effects of mAb RP/14. Two-color analysis of CD180 expression on splenic B lymphocytes. BALB/c splenocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553928, left panel) or biotinylated mAb RP/14 (right panel) in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142), followed by Streptavidin-PE (Cat. No. 554061) and FITC-conjugated anti-mouse CD45R/B220 mAb RA3-6B2 (Cat. No. 553087/553088). Flow cytometry was performed on a FACSCalibur™ .

BD Pharmingen™ Biotin Rat Anti-Mouse CD180 - Biotin - Clone RP/14 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: BALB/c mouse splenocytes

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD180 (Biotin) (ABIN2689038)

Ogata, Su, Miyake, Nagai, Akashi, Mecklenbräuker, Rajewsky, Kimoto, Tarakhovsky: "The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells." dans: The Journal of experimental medicine, Vol. 192, Issue 1, pp. 23-9, (2000) (PubMed).

Corcoran, Metcalf: "IL-5 and Rp105 signaling defects in B cells from commonly used 129 mouse substrains." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 11, pp. 5836-42, (1999) (PubMed).

Chan, Mecklenbräuker, Su, Texido, Leitges, Carsetti, Lowell, Rajewsky, Miyake, Tarakhovsky: "The molecular mechanism of B cell activation by toll-like receptor protein RP-105." dans: The Journal of experimental medicine, Vol. 188, Issue 1, pp. 93-101, (1998) (PubMed).

Miyake, Shimazu, Kondo, Niki, Akashi, Ogata, Yamashita, Miura, Kimoto: "Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1348-53, (1998) (PubMed).

Yamashita, Miyake, Miura, Kaneko, Yagita, Suda, Nagata, Nomura, Sakaguchi, Kimoto: "Activation mediated by RP105 but not CD40 makes normal B cells susceptible to anti-IgM-induced apoptosis: a role for Fc receptor coligation." dans: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 113-20, (1996) (PubMed).

Miyake, Yamashita, Ogata, Sudo, Kimoto: "RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3333-40, (1995) (PubMed).

Miyake, Yamashita, Hitoshi, Takatsu, Kimoto: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105-kD molecule: unresponsiveness of X-linked immunodeficient B cells." dans: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1217-24, (1994) (PubMed).

Détails sur CD180

Antigène: CD180 (CD180 Molecule (CD180))

Autre désignation: CD180

Sujet: Synonyms: RP105

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades