EpCAM anticorps

N° du produit ABIN2689192

Détail du produit anti-EpCAM anticorps

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Marque: BD Pharmingen™

Attributs du produit: The G8.8 antibody reacts with CD326/Ep-CAM (Epithelial Cell Adhesion Molecule), also known as gp40 in the mouse and by a variety of names (including GA733-2, CO17-1A, and EGP) in the human. In the mouse, Ep-CAM is a 40-42 kDa cell-surface glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, keratinocytes, Langerhans cells and lymph node and splenic dendritic cells. Profiles of Ep-CAM expression on fetal thymocytes and on the CD4[-] CD8[-], CD4[+] CD8[+], CD4[-] CD8[+], and CD4[+] CD8[-] subsets of adult thymocytes have been published. In unrelated studies, mouse Ep-CAM mRNA was detected in tissues containing epithelial cells (kidney, stomach, intestine, lung, and thymus) and in plasma cells and plasmacytomas, but not in heart, muscle, liver, brain, spleen, B lymphomas, or pre-B lymphomas. Ep-CAM is a Ca[2+] independent homophilic adhesion molecule which is proposed to play roles in the development and normal function of epithelial tissues and in the progression of carcinomas. Multicolor analysis of the expression of Ep- CAM on splenic T lymphocytes. BALB/c splenocytes were stained with FITC Hamster Anti-Mouse CD3e (Cat. No 553061/553062), PE Rat Anti-Mouse CD25 (Cat. No. 553866), and either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927, Left Panel) or Purified Rat Anti-Mouse CD326 (Cat. No. 552370, Right Panel) in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, Cat. No. 553141/553142). The G8.8 and isotype control mAb were detected by Biotin Mouse anti-Rat IgG2a (Cat. No. 553894), followed by Streptavidin-APC (Cat. No. 554067). Viable lymphocytes were gated for exclusion of propidium iodide, and the CD25+ subpopulation was selected for analysis of CD3e and Ep-CAM expression. A small population of CD3+CD25+Ep-CAM+ cells were detected (Right Panel), whereas the CD25- T cells do not express Ep-CAM (data not shown). Flow cytometry was performed on a BD FACSCalibur™

BD Pharmingen™ Purified Rat Anti-Mouse CD326 - Purified - Clone G8.8 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Glycoconjugates from BALB/c mouse-derived TE-71 medullary thymic epithelial cell line

Clone: G8-8

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-EpCAM (ABIN2689192)

Birebent, Somasundaram, Purev, Li, Mitchell, Hoey, Bloom, Mastrangelo, Maguire, Harris, Staib, Braumuller, Leeser, Kuttner, Beger, Herlyn: "Anti-idiotypic antibody and recombinant antigen vaccines in colorectal cancer patients." dans: Critical reviews in oncology/hematology, Vol. 39, Issue 1-2, pp. 107-13, (2001) (PubMed).

Taguchi, Hashimoto, Naiki, Farr, Boyd, Ansari, Shultz, Kotzin, Dorshkind, Ikehara, Gershwin: "Abnormal thymic expression of epithelial cell adhesion molecule (EP-CAM) in New Zealand Black (NZB) mice." dans: Journal of autoimmunity, Vol. 13, Issue 4, pp. 393-404, (2000) (PubMed).

Basak, Speicher, Eck, Wunner, Maul, Simmons, Herlyn: "Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue." dans: Journal of the National Cancer Institute, Vol. 90, Issue 9, pp. 691-7, (1998) (PubMed).

Zutter: "Gastrointestinal carcinoma antigen GA733: target for immunodestruction and potential modifier of invasiveness and chemoresponsiveness." dans: Journal of the National Cancer Institute, Vol. 90, Issue 9, pp. 642-4, (1998) (PubMed).

Litvinov, Balzar, Winter, Bakker, Briaire-de Bruijn, Prins, Fleuren, Warnaar: "Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins." dans: The Journal of cell biology, Vol. 139, Issue 5, pp. 1337-48, (1997) (PubMed).

Nelson, Dunn, Peach, Aruffo, Farr: "The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells." dans: European journal of immunology, Vol. 26, Issue 2, pp. 401-8, (1996) (PubMed).

Borkowski, Nelson, Farr, Udey: "Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells." dans: European journal of immunology, Vol. 26, Issue 1, pp. 110-4, (1996) (PubMed).

Bergsagel, Victor-Kobrin, Timblin, Trepel, Kuehl: "A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 2, pp. 590-6, (1992) (PubMed).

Farr, Nelson, Truex, Hosier: "Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 39, Issue 5, pp. 645-53, (1991) (PubMed).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: CD326 (EPCAM Produits)

Synonymes: anticorps DIAR5, anticorps EGP-2, anticorps EGP314, anticorps EGP40, anticorps ESA, anticorps HNPCC8, anticorps KS1/4, anticorps KSA, anticorps M4S1, anticorps MIC18, anticorps MK-1, anticorps TACSTD1, anticorps TROP1, anticorps ECS-1, anticorps ECS1, anticorps ESA1, anticorps M17S1, anticorps EGP, anticorps Ep-CAM, anticorps sb:cb6, anticorps tacstd, anticorps wu:fj17g02, anticorps zgc:110304, anticorps zgc:77119, anticorps tacstd1, anticorps MGC80540, anticorps EPCAM, anticorps egp, anticorps ksa, anticorps m4s1, anticorps mk-1, anticorps cd326, anticorps egp40, anticorps mic18, anticorps trop1, anticorps ep-cam, anticorps hegp-2, anticorps co17-1a, anticorps ga733-2, anticorps CD326, anticorps Egp314, anticorps EpCAM1, anticorps GA733-2, anticorps Ly74, anticorps Tacsd1, anticorps Tacstd1, anticorps gp40, anticorps epithelial cell adhesion molecule, anticorps flotillin 2, anticorps epithelial cell adhesion molecule S homeolog, anticorps EPCAM, anticorps FLOT2, anticorps epcam, anticorps epcam.S, anticorps Epcam

Sujet: Synonyms: Ep-CAM