CD45RB anticorps (Biotin)

N° du produit ABIN2689290

Détail du produit anti-CD45RB anticorps (Biotin)

Antigène: CD45RB

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RB est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Marque: BD Pharmingen™

Attributs du produit: The 16A antibody reacts with an exon B-dependent epitope of CD45 lycoprotein, which is found at high density on peripheral B cells, T cytotoxic/suppressor cells, subset of T helper cells, and most thymocytes and at low density on macrophages and dendritic cells.The level of CD45RB expression appears to decrease as T lymphocytes progress from naive to memory cells. In addition, subpopulations of CD4+ T cells which express high and low levels of D45RB have different ytokine secretion profiles and mediate distinct immunological functions. CD25+ D4+ regulatory T (Treg) lymphocytes which control intestinal inflammation and autoimmunity express low levels of D45RB. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD45RB - Biotin - Clone 16A - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 16A

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD45RB (Biotin) (ABIN2689290)

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." dans: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, (2000) (PubMed).

Ernst, Weigle, Noonan, McQuitty, Hobbs: "The age-associated increase in IFN-gamma synthesis by mouse CD8+ T cells correlates with shifts in the frequencies of cell subsets defined by membrane CD44, CD45RB, 3G11, and MEL-14 expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 2, pp. 575-87, (1993) (PubMed).

Inoue, Asano, Matsuoka, Furutani-Seiki, Aizawa, Nishimura, Shirai, Tada: "Distinction of mouse CD8+ suppressor effector T cell clones from cytotoxic T cell clones by cytokine production and CD45 isoforms." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 6, pp. 2121-8, (1993) (PubMed).

Rogers, Pilapil, Hayakawa, Romain, Parker: "CD45 alternative exon expression in murine and human CD4+ T cell subsets." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 4054-65, (1992) (PubMed).

Hathcock, Laszlo, Dickler, Sharrow, Johnson, Trowbridge, Hodes: "Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 1, pp. 19-28, (1992) (PubMed).

Dianzani, Luqman, Rojo, Yagi, Baron, Woods, Janeway, Bottomly: "Molecular associations on the T cell surface correlate with immunological memory." dans: European journal of immunology, Vol. 20, Issue 10, pp. 2249-57, (1990) (PubMed).

Bottomly, Luqman, Greenbaum, Carding, West, Pasqualini, Murphy: "A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines." dans: European journal of immunology, Vol. 19, Issue 4, pp. 617-23, (1989) (PubMed).

Johnson, Greenbaum, Bottomly, Trowbridge: "Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies." dans: The Journal of experimental medicine, Vol. 169, Issue 3, pp. 1179-84, (1989) (PubMed).

Détails sur CD45RB

Antigène: CD45RB

Autre désignation: CD45RB (CD45RB Produits)

Synonymes: anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps PTPRC, anticorps Ptprc