CD5 anticorps

N° du produit ABIN2689320

Il existe 18+ publications pour ce produit. L’anticorps anti-CD5 Monoclonal Rat est utilisé pour la détection de CD5 dans des échantillons de Souris. Il a été validé pour FACS, IP, ELISA, IHC (fro), IHC (f), CyTox et BR.

Aperçu rapide pour CD5 anticorps (ABIN2689320)

Antigène: CD5

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Cytotoxicity Test (CyTox), Blocking Reagent (BR)

Clone: 53

Détail du produit anti-CD5 anticorps

Attributs du produit: The 53-7.3 clone has been reported to react with a monomorphic epitope of CD5, a member of the scavenger receptor cysteine-rich protein superfamily and the major ligand of CD72, found on thymocytes, T lymphocytes, thymic NK-T cells, and a subset of B lymphocytes, but not on NK cells or splenic NK-T cells. The level of surface CD5 expression is developmentally regulated in the thymus, starting with low levels on CD4-CD8- thymocytes and increasing as they mature to CD4+CD8+ then CD4+CD8- or CD4-CD8+ thymocytes. Relatively high levels are maintained on peripheral T lymphocytes. The level of CD5 antigen detected on T helper cells has been reported to be somewhat higher than that on T cytotoxic/suppressor and B cells. Few, if any, intestinal intraepithelial lymphocytes bearing the γδ T-cell receptor express CD5. Phenotypic, anatomical, functional, developmental, and pathogenic characteristics of peripheral CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. The frequency of these CD5+ B cells has been reported to show strain-dependent variation. An additional population of CD5+ B lymphocytes resides in the thymus, where it matures from intrathymic B-cell progenitors. It has been proposed that CD5 is a costimulatory molecule which mediates interactions of cells in the immune system and negatively regulates signal transduction mediated by the T-cell receptor and B-cell receptor. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD5 - Purified - Clone 53-7.3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Thymus / Spleen

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD5 (ABIN2689320)

Lanier: "Natural killer cell receptor signaling." dans: Current opinion in immunology, Vol. 15, Issue 3, pp. 308-14, (2003) (PubMed).

Azzam, Grinberg, Lui, Shen, Shores, Love: "CD5 expression is developmentally regulated by T cell receptor (TCR) signals and TCR avidity." dans: The Journal of experimental medicine, Vol. 188, Issue 12, pp. 2301-11, (1999) (PubMed).

Bikah, Lynd, Aruffo, Ledbetter, Bondada: "A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5." dans: International immunology, Vol. 10, Issue 8, pp. 1185-96, (1998) (PubMed).

Yashiro, Tai, Toyo-oka, Park, Abe, Hamaoka, Kobayashi, Neben, Fujiwara: "A fundamental difference in the capacity to induce proliferation of naive T cells between CD28 and other co-stimulatory molecules." dans: European journal of immunology, Vol. 28, Issue 3, pp. 926-35, (1998) (PubMed).

Bendelac, Rivera, Park, Roark: "Mouse CD1-specific NK1 T cells: development, specificity, and function." dans: Annual review of immunology, Vol. 15, pp. 535-62, (1997) (PubMed).

Bikah, Carey, Ciallella, Tarakhovsky, Bondada: "CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells." dans: Science (New York, N.Y.), Vol. 274, Issue 5294, pp. 1906-9, (1997) (PubMed).

Masuda, Makino, Cui, Ito, Tokuhisa, Takahama, Koseki, Tsuchida, Koike, Moriya, Amano, Taniguchi: "Phenotypes and invariant alpha beta TCR expression of peripheral V alpha 14+ NK T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 5, pp. 2076-82, (1997) (PubMed).

Mori, Inaba, Sugihara, Taketani, Doi, Fukuba, Yamamoto, Adachi, Inaba, Fukuhara, Ikehara: "Presence of B cell progenitors in the thymus." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 9, pp. 4193-9, (1997) (PubMed).

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." dans: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Tarakhovsky, Kanner, Hombach, Ledbetter, Müller, Killeen, Rajewsky: "A role for CD5 in TCR-mediated signal transduction and thymocyte selection." dans: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 535-7, (1995) (PubMed).

Kantor, Herzenberg: "Origin of murine B cell lineages." dans: Annual review of immunology, Vol. 11, pp. 501-38, (1993) (PubMed).

Luo, Van de Velde, von Hoegen, Parnes, Thielemans: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, (1992) (PubMed).

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).

Hayakawa, Hardy: "Normal, autoimmune, and malignant CD5+ B cells: the Ly-1 B lineage?" dans: Annual review of immunology, Vol. 6, pp. 197-218, (1988) (PubMed).

Hayakawa, Hardy, Parks, Herzenberg: "The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice." dans: The Journal of experimental medicine, Vol. 157, Issue 1, pp. 202-18, (1983) (PubMed).

van Ewijk, van Soest, van den Engh: "Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2, and T-200." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2594-604, (1982) (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." dans: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." dans: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).

Détails sur CD5

Antigène: CD5

Autre désignation: CD5

Sujet: Synonyms: Ly-1