ICAM1 anticorps

N° du produit ABIN2689330

Détail du produit anti-ICAM1 anticorps

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM1 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Marque: BD Pharmingen™

Attributs du produit: The 3E2 antibody reacts with CD54 (ICAM-1), a 95- kDa member of the Ig superfamily found on lymphocytes, vascular endothelium, high endothelial venules, epithelial cells, macrophages, and dendritic cells. ICAM-1 is a ligand for LFA1 (CD11a/CD18) and Mac-1 (CD11b/CD18). Its expression is upregulated upon stimulation by inflammatory mediators such as cytokines and LPS. Studies with mouse Icam1-transfected antigen-presenting cells, with CD54-blocking antibodies, and in CD54-deficient mice indicate that CD54 participates in inflammatory reactions and antigen-specific immune responses. In addition, there is evidence that CD54 is a receptor involved in MHC-non-restricted responses to weakly immunogenic tumor cells. The 3E2 antibody blocks in vitro and in responses. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel: Naive BALB/c splenocytes were stained with purified 3E2 mAb (open histogram) followed by FITC-conjugated anti-hamster IgG cocktail (Cat. No. 554011, filled and open histograms). Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD (BD Via-Probe™, Cat. No. 555816/555815). The mean fluorescence intensity of the stained lymphocytes is about 7.5 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with purified 3E2 mAb (open histogram) followed by FITC-conjugated anti-hamster IgG (filled and open histograms). Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 15 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Hamster Anti-Mouse CD54 - Purified - Clone 3E2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Not reported

Clone: 3E2

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-ICAM1 (ABIN2689330)

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." dans: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Gonzalo, Martinez, Springer, Gutierrez-Ramos: "ICAM-1 is required for T cell proliferation but not for anergy or apoptosis induced by Staphylococcus aureus enterotoxin B in vivo." dans: International immunology, Vol. 7, Issue 10, pp. 1691-8, (1996) (PubMed).

Kelly, Williams, Colvin, Meehan, Springer, Gutierrez-Ramos, Bonventre: "Intercellular adhesion molecule-1-deficient mice are protected against ischemic renal injury." dans: The Journal of clinical investigation, Vol. 97, Issue 4, pp. 1056-63, (1996) (PubMed).

Nishio, Podack: "Rapid induction of tumor necrosis factor cytotoxicity in naive splenic T cells by simultaneous CD80 (B7.1) and CD54 (ICAM-1) co-stimulation." dans: European journal of immunology, Vol. 26, Issue 9, pp. 2160-4, (1996) (PubMed).

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, (1996) (PubMed).

Soriano, Lipton, Wang, Xiao, Springer, Gutierrez-Ramos, Hickey: "Intercellular adhesion molecule-1-deficient mice are less susceptible to cerebral ischemia-reperfusion injury." dans: Annals of neurology, Vol. 39, Issue 5, pp. 618-24, (1996) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." dans: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Xu, Gonzalo, St Pierre, Williams, Kupper, Cotran, Springer, Gutierrez-Ramos: "Leukocytosis and resistance to septic shock in intercellular adhesion molecule 1-deficient mice." dans: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 95-109, (1994) (PubMed).

Isobe, Yagita, Okumura, Ihara: "Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1." dans: Science (New York, N.Y.), Vol. 255, Issue 5048, pp. 1125-7, (1992) (PubMed).

Siu, Hedrick, Brian: "Isolation of the murine intercellular adhesion molecule 1 (ICAM-1) gene. ICAM-1 enhances antigen-specific T cell activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 11, pp. 3813-20, (1990) (PubMed).

Springer: "Adhesion receptors of the immune system." dans: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).

Détails sur ICAM1

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: CD54 (ICAM1 Produits)

Synonymes: anticorps BB2, anticorps CD54, anticorps P3.58, anticorps Icam-1, anticorps Ly-47, anticorps MALA-2, anticorps ICAM, anticorps ICAM-1, anticorps ICAM1, anticorps intercellular adhesion molecule 1, anticorps intercellular adhesion molecule-1, anticorps ICAM1, anticorps Icam1, anticorps ICAM-1

Classe de substances: Viral Protein

Sujet: Synonyms: ICAM-1

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling