Selectin E/CD62e anticorps (Biotin)

N° du produit ABIN2689358

Détail du produit anti-Selectin E/CD62e anticorps (Biotin)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The 10E9.6 antibody reacts with the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells. A suspension of TNFα stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen. The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNFα-treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD62E - Biotin - Clone 10E9.6 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse brain capillary endothelioma bEnd.3 (TNFalpha-stimulated) Cell Line

Clone: 10E9-6

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Selectin E/CD62e (Biotin) (ABIN2689358)

Pendl, Robert, Steinert, Thanos, Eytner, Borges, Wild, Lowe, Fuhlbrigge, Kupper, Vestweber, Grabbe: "Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1." dans: Blood, Vol. 99, Issue 3, pp. 946-56, (2002) (PubMed).

Borges, Pendl, Eytner, Steegmaier, Zöllner, Vestweber: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." dans: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).

Ramos, Kunkel, Lawrence, Jung, Vestweber, Bosse, McIntyre, Gillooly, Norton, Wolitzky, Ley: "Differential effect of E-selectin antibodies on neutrophil rolling and recruitment to inflammatory sites." dans: Blood, Vol. 89, Issue 8, pp. 3009-18, (1997) (PubMed).

Eppihimer, Wolitzky, Anderson, Labow, Granger: "Heterogeneity of expression of E- and P-selectins in vivo." dans: Circulation research, Vol. 79, Issue 3, pp. 560-9, (1996) (PubMed).

Bosse, Vestweber: "Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum." dans: European journal of immunology, Vol. 24, Issue 12, pp. 3019-24, (1995) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." dans: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Weller, Isenmann, Vestweber: "Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha." dans: The Journal of biological chemistry, Vol. 267, Issue 21, pp. 15176-83, (1992) (PubMed).

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: CD62E (SELE Produits)

Synonymes: anticorps SELE, anticorps CD62E, anticorps ELAM, anticorps ELAM1, anticorps ESEL, anticorps LECAM2, anticorps E-selectin, anticorps Elam, anticorps selectin E, anticorps selectin, endothelial cell, anticorps SELE, anticorps Sele

Sujet: Synonyms: E-selectin, ELAM-1

Pathways: Thromboxane A2 Receptor Signaling