CD81 anticorps (Biotin)

N° du produit ABIN2689414

Détail du produit anti-CD81 anticorps (Biotin)

Antigène: CD81

Reactivité: Souris, Rat

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD81 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The Eat2 antibody reacts with CD81, a 26- kDa nonglycosylated member of the transmembrane 4 integral membrane protein superfamily, expressed by many types of cells. For example, CD81 participates with CD19 and CD21 in the signal transduction complex associated with the B-cell receptor on human B lymphocytes and with the CD4 and CD8 co-receptors on human thymocytes and T lymphocytes. In mouse fetal thymic organ culture, interactions of immature thymocytes with CD81 expressed by thymic stromal cells are required to induce development of T cells with αβ T-cell receptors. Furthermore, CD81 has been shown to play a role in the regulation of rat mastcell degranulation. Despite its important roles in the immune response and wide tissue distribution, CD81-deficient mice are born without obvious developmental abnormalities. However, these mice have abnormal immune responses, and impaired fertility. Eat2 mAb cross-reacts with the rat CD81 antigen. Two-color analysis of the expression of CD81 on mouse and rat splenocytes. C57BL/6 splenocytes were simultaneously stained with FITC Rat anti-Mouse CD45R/B220 (Cat. no. 553087/553088, First and second panels) and PE Hamster IgG1 κ Isotype Control (Cat. no. 553972, First panel) or PE Hamster Anti-Mouse/Rat CD81 (Cat. No, 559519, Second panel) monoclonal antibodies. Similarly, LOU splenocytes were simultaneously stained with FITC Mouse anti-Rat CD45RA (Cat. No. 554883, Third and last panels) and PE Hamster IgG1 κ Isotype Control (Cat. no. 553972, Third panel) or PE Hamster anti-Mouse/Rat CD81 (Cat. No, 559519, Last panel) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ Flow Cytometry System (BD Biosciences,San Jose, CA

BD Pharmingen™ Biotin Hamster Anti-Mouse/Rat CD81 - Biotin - Clone Eat2 - Isotype Armenian Hamster IgG, κ - Reactivity Ms, Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: CD81+ mouse B lymphoma 38C13

Clone: Eat2

Isotype: IgG kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD81 (Biotin) (ABIN2689414)

Deng, Yeung, Tsitoura, DeKruyff, Umetsu, Levy: "Allergen-induced airway hyperreactivity is diminished in CD81-deficient mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5054-61, (2000) (PubMed).

Maecker, Todd, Kim, Levy: "Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies." dans: Hybridoma, Vol. 19, Issue 1, pp. 15-22, (2000) (PubMed).

Maecker, Do, Levy: "CD81 on B cells promotes interleukin 4 secretion and antibody production during T helper type 2 immune responses." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 5, pp. 2458-62, (1998) (PubMed).

Levy, Todd, Maecker: "CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system." dans: Annual review of immunology, Vol. 16, pp. 89-109, (1998) (PubMed).

Fleming, Donnadieu, Song, Laethem, Galli, Kinet: "Negative regulation of Fc epsilon RI-mediated degranulation by CD81." dans: The Journal of experimental medicine, Vol. 186, Issue 8, pp. 1307-14, (1997) (PubMed).

Maecker, Levy: "Normal lymphocyte development but delayed humoral immune response in CD81-null mice." dans: The Journal of experimental medicine, Vol. 185, Issue 8, pp. 1505-10, (1997) (PubMed).

Miyazaki, Müller, Campbell: "Normal development but differentially altered proliferative responses of lymphocytes in mice lacking CD81." dans: The EMBO journal, Vol. 16, Issue 14, pp. 4217-25, (1997) (PubMed).

Tsitsikov, Gutierrez-Ramos, Geha: "Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 20, pp. 10844-9, (1997) (PubMed).

Boismenu, Rhein, Fischer, Havran: "A role for CD81 in early T cell development." dans: Science (New York, N.Y.), Vol. 271, Issue 5246, pp. 198-200, (1996) (PubMed).

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." dans: Immunology today, Vol. 15, Issue 9, pp. 437-42, (1994) (PubMed).

Détails sur CD81

Antigène: CD81

Autre désignation: CD81 (CD81 Produits)

Synonymes: anticorps Tapa-1, anticorps Tapa1, anticorps Tspan28, anticorps CVID6, anticorps S5.7, anticorps TAPA1, anticorps TSPAN28, anticorps CD81 molecule, anticorps CD81 molecule b, anticorps CD81 antigen, anticorps Cd81 molecule, anticorps CD81, anticorps cd81b, anticorps Cd81

Sujet: Synonyms: TAPA-1, Tspan28, Tapa1, Tapa-1, Target of the antiproliferative antibody 1,

Pathways: Inositol Metabolic Process, Hepatitis C