CD90.2 / Thy-1.2 anticorps

N° du produit ABIN2689454

Cet anticorps Rat Monoclonal détecte spécifiquement CD90.2 / Thy-1.2 dans FACS, IP, IHC (fro) et CyTox. Il présente une réactivité envers Souris et a été mentionné dans 13+ publications.

Aperçu rapide pour CD90.2 / Thy-1.2 anticorps (ABIN2689454)

Antigène: CD90.2 / Thy-1.2

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD90.2 / Thy-1.2 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Clone: 53

Détail du produit anti-CD90.2 / Thy-1.2 anticorps

Marque: BD Pharmingen™

Attributs du produit: The 53-2.1 antibody reacts with the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. mAb 53-2.1 has been reported not to cross-react with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. mAb 53-2.1 has been reported to block the binding of anti-mouse CD90.2 clone 30-H12 (Cat. No. 553009) to immobilized thymocyte membranes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD90.2 - Purified - Clone 53-2.1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Thymus / Spleen

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry : Mouse BD Fc Block™ purified anti-mouse CD16/CD32 clone 2.4G2 may help to reduce non-specific binding to cells bearing Fcγ-receptors. For staining in the presence of Mouse BD Fc Block™, a second step antibody which does not recognize the 2.4G2 mAb (Rat IgG2b, κ) must be used, such as FITC anti-rat IgG2a, mAb RG7/1.30 . Caution: Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use. Page 1 of 2553000 Rev. 12

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD90.2 / Thy-1.2 (ABIN2689454)

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." dans: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).

Zhong, Donnenberg, Edison, Harrison: "The appearance of Thy-1- donor T cells in the peripheral circulation 3-6 weeks after bone marrow transplantation suggests an extrathymic origin." dans: International immunology, Vol. 8, Issue 2, pp. 171-6, (1996) (PubMed).

Borrello, Phipps: "Differential Thy-1 expression by splenic fibroblasts defines functionally distinct subsets." dans: Cellular immunology, Vol. 173, Issue 2, pp. 198-206, (1996) (PubMed).

Radrizzani, Carminatti, Pivetta, Idoyaga Vargas: "Developmental regulation of Thy 1.2 rate of synthesis in the mouse cerebellum." dans: Journal of neuroscience research, Vol. 42, Issue 2, pp. 220-7, (1996) (PubMed).

Hueber, Raposo, Pierres, He: "Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism." dans: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 785-96, (1994) (PubMed).

Ikuta, Uchida, Friedman, Weissman: "Lymphocyte development from stem cells." dans: Annual review of immunology, Vol. 10, pp. 759-83, (1992) (PubMed).

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" dans: Immunology today, Vol. 12, Issue 12, pp. 436-8, (1992) (PubMed).

He, Naquet, Caillol, Pierres: "Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism." dans: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 515-8, (1991) (PubMed).

Tigelaar, Lewis, Bergstresser: "TCR gamma/delta+ dendritic epidermal T cells as constituents of skin-associated lymphoid tissue." dans: The Journal of investigative dermatology, Vol. 94, Issue 6 Suppl, pp. 58S-63S, (1990) (PubMed).

Kroczek, Gunter, Germain, Shevach: "Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes." dans: Nature, Vol. 322, Issue 6075, pp. 181-4, (1986) (PubMed).

Williams, Gagnon: "Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin." dans: Science (New York, N.Y.), Vol. 216, Issue 4547, pp. 696-703, (1982) (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." dans: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." dans: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).

Détails sur CD90.2 / Thy-1.2

Antigène: CD90.2 / Thy-1.2

Autre désignation: CD90.2

Sujet: Synonyms: Thy-1.2