Disialoganglioside GD3 anticorps

N° du produit ABIN2689491

Il existe 5+ publications pour ce produit. L’anticorps anti-Disialoganglioside GD3 Monoclonal Souris est utilisé pour la détection de Disialoganglioside GD3 dans des échantillons de Humain. Il a été validé pour CyTox, IHC (fro), FACS et IF.

Aperçu rapide pour Disialoganglioside GD3 anticorps (ABIN2689491)

Antigène: Disialoganglioside GD3

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MB3-6

Détail du produit

Attributs du produit: Gangliosides are sialic-acid bearing glycolipids expressed on the surface of all mammalian cells, and are likely involved in mediating cell-substratum interactions. They are important target antigens for antibody mediated cytolysis of human melanoma and neuroblastoma cells. Human melanoma cells produce gangliosides GD2 and/or GD3 which are present in substratum-attached material, and may play a significant role in the melanoma metastatic phenotype. Ganglioside GD3 is a major surface marker on most human melanoma cells. MB3.6 has been used to localize GD3 in the plasma membrane and in focal adhesion plaques of human melanoma cells.

BD Pharmingen™ Purified Mouse Anti-Human Disialoganglioside GD3 - Purified - Clone MB3.6 - Isotype Mouse IgG3, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: FM9 human melamona cell line

Isotype: IgG3 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Référence

Mujoo, Cheresh, Yang, Reisfeld: "Disialoganglioside GD2 on human neuroblastoma cells: target antigen for monoclonal antibody-mediated cytolysis and suppression of tumor growth." dans: Cancer research, Vol. 47, Issue 4, pp. 1098-104, (1987) (PubMed).

Cheresh, Pierschbacher, Herzig, Mujoo: "Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins." dans: The Journal of cell biology, Vol. 102, Issue 3, pp. 688-96, (1986) (PubMed).

Cheresh, Honsik, Staffileno, Jung, Reisfeld: "Disialoganglioside GD3 on human melanoma serves as a relevant target antigen for monoclonal antibody-mediated tumor cytolysis." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 15, pp. 5155-9, (1985) (PubMed).

Hakomori: "Tumor-associated carbohydrate antigens." dans: Annual review of immunology, Vol. 2, pp. 103-26, (1985) (PubMed).

Cheresh, Harper, Schulz, Reisfeld: "Localization of the gangliosides GD2 and GD3 in adhesion plaques and on the surface of human melanoma cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 18, pp. 5767-71, (1984) (PubMed).

Détail du antigène

Antigène: Disialoganglioside GD3