Fetal Hemoglobin anticorps

N° du produit ABIN2689504

Cité dans 3 publications. Cet anticorps anti-Fetal Hemoglobin Monoclonal Souris (Clone 2D12) (ABIN2689504) détecte spécifiquement Fetal Hemoglobin dans FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Fetal Hemoglobin anticorps (ABIN2689504)

Antigène: Fetal Hemoglobin (HbF)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fetal Hemoglobin est non-conjugé

Application: Flow Cytometry (FACS)

Clone: 2D12

Détail du produit anti-Fetal Hemoglobin anticorps

Attributs du produit: Reacts with fetal hemoglobin (HbF), a form of hemoglobin present during fetal development. HbF resembles adult hemoglobin (HbA) in possessing two alpha chains but differs in possessing two gamma instead of two beta chains. In normal adults, synthesis of HbF does persist at very low levels (<1 % of total Hb) and is restricted to a small population of erythrocytes called F cells. Hemoglobin F-expressing erythrocytes are normally seen during pregnancy. An increase in the expression of fetal hemoglobin in adult peripheral red blood cells is a common feature in the genetic disorders of hemoglobin, sickle-cell disease (SCD) and beta thalassemia. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of erythrocytes obtained from cord blood analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human Fetal Hemoglobin - Purified - Clone 2D12 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Fetal Hemoglobin (ABIN2689504)

Campbell, Ware, Mason: "Detection of hemoglobin variants in erythrocytes by flow cytometry." dans: Cytometry, Vol. 35, Issue 3, pp. 242-8, (1999) (PubMed).

Horiuchi, Osterhout, Kamma, Bekoe, Hirokawa: "Estimation of fetal hemoglobin levels in individual red cells via fluorescence image cytometry." dans: Cytometry, Vol. 20, Issue 3, pp. 261-7, (1995) (PubMed).

Thorpe, Thein, Sampietro, Craig, Mahon, Huehns: "Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis." dans: British journal of haematology, Vol. 87, Issue 1, pp. 125-32, (1994) (PubMed).

Détails sur Fetal Hemoglobin

Antigène: Fetal Hemoglobin (HbF)

Autre désignation: Fetal Hemoglobin