MHC Class I (H-2Kb/H-2Db) anticorps (Biotin)

N° du produit ABIN2689543

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans FACS. Adapté pour Souris. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour MHC Class I (H-2Kb/H-2Db) anticorps (Biotin) (ABIN2689543)

Antigène: MHC Class I (H-2Kb/H-2Db)

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS)

Clone: 28-8

Détail du produit

Marque: BD Pharmingen™

Attributs du produit: The 28-8-6 antibody reacts with the H-2K[b] and H-2D[b] MHC class I alloantigens. The reaction with H-2K[b] has been reported to be stronger than that with H-2D[b]. Its recognition of H-2D[b] is dependent upon the presence of β2 microglobulin. Cross-reactivity with an epitope on the N-terminal domains, α1 and α2, of the H-2D[d] MHC class I alloantigen has also been described. Reactivity with other haplotypes (e.g., f, k, p, q, r, s) has not been observed.

BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b]/H-2D[b] - Biotin - Clone 28-8-6 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: C3H.SW mouse splenocytes

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Référence

Allen, Fraser, Flyer, Calvin, Flavell: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).

Evans, Margulies, Shykind, Seidman, Ozato: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." dans: Nature, Vol. 300, Issue 5894, pp. 755-7, (1983) (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).

Détail du antigène

Antigène: MHC Class I (H-2Kb/H-2Db)

Autre désignation: H-2K[b]/H-2D[b]