IL-10 anticorps

N° du produit ABIN2689722

L’anticorps Rat Monoclonal anti-IL-10 a été validé pour ELISA, Neut et Inhibition. Il convient pour détecter IL-10 dans des échantillons de Humain et Virus. Il y a 2+ publications disponibles.

Aperçu rapide pour IL-10 anticorps (ABIN2689722)

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivité: Humain, Virus

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-10 est non-conjugé

Application: ELISA, Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: JES3

Détail du produit anti-IL-10 anticorps

Marque: BD Pharmingen™

Attributs du produit: The JES3-19F1 antibody reacts with human (IL-10). The immunogen used to generate the JES3-19F1 hybridoma was recombinant human IL-10 expressed in COS cells. This is a neutralizing antibody. This antibody also reacts with viral IL-10. Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hours with LPS (1.0 μg/mL) in the presence of GolgiStop™ (Cat. No. 554724, 2 μM final concentration). The PBMC were harvested, stained with FITC-mouse anti-human CD14 antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and subsequently stained with 0.25 μg of PE-rat anti-human IL-10 antibody (PE-JES3-19F1, Cat. No. 554706) following Pharmingen's staining protocol (see image, left panel). The data reflects gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of the PE-JES3-19F1 antibody was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 (0.5 mg, Cat. No. 554611, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled JES3-19F1 antibody (10 mg, Cat. No. 554704, right panel) prior to staining with the PE-JES3-19F1 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody (right panel) blocking specificity controls.

BD Pharmingen™ Purified Rat Anti-Human IL-10 - Purified - Clone JES3-19F1 - Isotype Rat IgG2a - Reactivity Hu, Vir - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant Human IL-10

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IL-10 (ABIN2689722)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Andersson, Christensen, Marker, Thomsen: "Changes in cell adhesion molecule expression on T cells associated with systemic virus infection." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1237-45, (1994) (PubMed).

Détails sur IL-10

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: IL-10

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints