Interleukin 17a anticorps

N° du produit ABIN2689741

Cet anticorps anti-Interleukin 17a est un anticorps Rat Monoclonal détectant Interleukin 17a dans ELISA, WB, Neut et Inhibition. Adapté pour Souris. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour Interleukin 17a anticorps (ABIN2689741)

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Interleukin 17a est non-conjugé

Application: ELISA, Western Blotting (WB), Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: TC11

Détail du produit anti-Interleukin 17a anticorps

Marque: BD Pharmingen™

Attributs du produit: The TC11-18H10 antibody reacts with recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro, it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRαβ+CD4-CD8-thymocytes. Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug™ (1 μg/mL final concentration, Cat. No. 555029) without (left panel) or with PMA (5 ng/mL final concentration, Sigma, Cat. No. P-8139) and Ionomycin (500 ng/mL final concentration, Sigma, Cat. No.I-0634) (middle and right panels). The cells were fixed, permeabilized and subsequently stained with 0.125 μg of PE conjugated anti-mouse IL-17A (PE-TC11-18H10, Cat. No.559502) using BD Pharmingen I/C staining protocol (left and middle panels) . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody (10 μg, right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained (left panel) and unlabeled antibody blocking (right panel) controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-17A - Purified - Clone TC11-18H10 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant Mouse IL-17 Protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Interleukin 17a (ABIN2689741)

Schwarzenberger, La Russa, Miller, Ye, Huang, Zieske, Nelson, Bagby, Stoltz, Mynatt, Spriggs, Kolls: "IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6383-9, (1998) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Détails sur Interleukin 17a

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Autre désignation: IL-17A