The MM17F8F5 monoclonal antibody specifically binds to Interleukin-17F (IL-17F) and can neutralize its biological activity. IL-17F is a member of the IL-17 family of cytokines. Among IL-17 family members, IL-17F has the highest amino acid sequence homology to IL-17A. IL-17F is produced by activated CD4+ T helper (Th17) cells, CD8+ T (Tc17) cells and γδ T cells. IL-17F can be secreted as homodimers or as heterodimers with IL-17A. IL-17F and IL-17A have overlapping functions such as inducing epithelial cells and fibroblasts to produce proinflammatory cytokines and chemokines including IL-6, GM-CSF, CXCL1, CCL2, and CCL7. These factors attract and activate neutrophils and other cell types that mediate protective responses against pathogenic microbes or pathologic allergic or autoimmune diseases. IL-17 gene knockout studies have shown that IL-17F and IL-17A have independent functions as well. IL-17F and IL-17A exert their biological function by binding to and signaling through IL-17 receptors comprised of the transmembrane receptor subunits, IL-17RA (CD217) and IL-17RC. Neutralization of mouse IL-17F biological activity with MM17F8F5, a Mouse Anti-Mouse IL-17F monoclonal antibody. In a 96-well microplate, biologically-active recombinant mouse IL-17F protein (0.2 μg/well) was preincubated (1 hr, 37 °C) with serial dilutions of Purified NA/LE Mouse Anti-Mouse IL-17F antibody (Cat. No. 563592, μg/mL) or tissue culture medium (as a control). Following the incubation, cells from the NIH-3T3 mouse embryonic cell line were added at 5 x 10^4 cells per well and were cultured at 37 °C. After 24 hours of culture, the supernatants from each well were harvested and their concentrations of IL-6 were quantified using a BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301). As the MM17F8F5 antibody was serially diluted, a neutralizing Anti-Mouse IL-17F antibody dose-response relationship was observed as shown in the figure. 563592 Rev. 1 Page 1 of 2
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Agent conservateur
Without preservative
Conseil sur la manipulation
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Wilhelm, Hirota, Stieglitz, Van Snick, Tolaini, Lahl, Sparwasser, Helmby, Stockinger: "An IL-9 fate reporter demonstrates the induction of an innate IL-9 response in lung inflammation." dans: Nature immunology, Vol. 12, Issue 11, pp. 1071-7, (2011) (PubMed).
Chang, Dong: "IL-17F: regulation, signaling and function in inflammation." dans: Cytokine, Vol. 46, Issue 1, pp. 7-11, (2009) (PubMed).
Martinez, Zhang, Chung, Reynolds, Lin, Jetten, Feng, Dong: "Smad3 differentially regulates the induction of regulatory and inflammatory T cell differentiation." dans: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35283-6, (2009) (PubMed).
Sutton, Lalor, Sweeney, Brereton, Lavelle, Mills: "Interleukin-1 and IL-23 induce innate IL-17 production from gammadelta T cells, amplifying Th17 responses and autoimmunity." dans: Immunity, Vol. 31, Issue 2, pp. 331-41, (2009) (PubMed).
Dong: "Mouse Th17 cells: current understanding of their generation and regulation." dans: European journal of immunology, Vol. 39, Issue 3, pp. 640-4, (2009) (PubMed).
Hamada, Garcia-Hernandez, Reome, Misra, Strutt, McKinstry, Cooper, Swain, Dutton: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, (2009) (PubMed).
Ishigame, Kakuta, Nagai, Kadoki, Nambu, Komiyama, Fujikado, Tanahashi, Akitsu, Kotaki, Sudo, Nakae, Sasakawa, Iwakura: "Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses." dans: Immunity, Vol. 30, Issue 1, pp. 108-19, (2009) (PubMed).
Yang, Chang, Park, Nurieva, Shah, Acero, Wang, Schluns, Broaddus, Zhu, Dong: "Regulation of inflammatory responses by IL-17F." dans: The Journal of experimental medicine, Vol. 205, Issue 5, pp. 1063-75, (2008) (PubMed).
Oda, Canelos, Essayan, Plunkett, Myers, Huang: "Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response." dans: American journal of respiratory and critical care medicine, Vol. 171, Issue 1, pp. 12-8, (2004) (PubMed).