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Sca-1/Ly-6A/E anticorps

Ly6a Reactivité: Souris FACS, IP, IHC (fro), CyTox Hôte: Rat Monoclonal E13 unconjugated
N° du produit ABIN2689819
  • Antigène Voir toutes Sca-1/Ly-6A/E (Ly6a) Anticorps
    Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
    Reactivité
    • 74
    • 6
    • 1
    Souris
    Hôte
    • 53
    • 26
    Rat
    Clonalité
    • 58
    • 17
    Monoclonal
    Conjugué
    • 18
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Sca-1/Ly-6A/E est non-conjugé
    Application
    • 50
    • 22
    • 20
    • 14
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)
    Marque
    BD Pharmingen™
    Attributs du produit
    The E13-161.7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca1 (Ly-6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cell (HSC) in mice with both Ly-6 haplotypes. Sca-1+ HSC are found in the adult bone marrow and fetal liver, but not in the early embryo yolk sac or intraembryonic hematopoietic sites, and can be mobilized to the peripheral blood and spleen in the adult. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, on the earliest intrathymic T-cell precursor population, and in several non-hematopoietic tissues. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody (Cat. No. 557403) have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb can block binding of FITC-conjugated D7 antibody (anti-Ly-6A/E, Cat. No. 557405) to mouse splenocytes, but purified mAb D7 (Cat. No. 557403) is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 559971) to identify HSC. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

    BD Pharmingen™ Purified Rat Anti-Mouse Ly-6A/E - Purified - Clone E13-161.7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    BALB/c mouse-derived "pre-T" cell hybridoma
    Clone
    E13
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product Ly6a Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Kawamoto, Ohmura, Katsura: "Direct evidence for the commitment of hematopoietic stem cells to T, B and myeloid lineages in murine fetal liver." dans: International immunology, Vol. 9, Issue 7, pp. 1011-9, (1997) (PubMed).

    Morrison, Wandycz, Hemmati, Wright, Weissman: "Identification of a lineage of multipotent hematopoietic progenitors." dans: Development (Cambridge, England), Vol. 124, Issue 10, pp. 1929-39, (1997) (PubMed).

    Osawa, Nakamura, Nishi, Takahasi, Tokuomoto, Inoue, Nakauchi: "In vivo self-renewal of c-Kit+ Sca-1+ Lin(low/-) hemopoietic stem cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 9, pp. 3207-14, (1996) (PubMed).

    Yamamoto, Yasumizu, Amou, Watanabe, Nishio, Toki, Fukuhara, Ikehara: "Characterization of peripheral blood stem cells in mice." dans: Blood, Vol. 88, Issue 2, pp. 445-54, (1996) (PubMed).

    Auerbach, Huang, Lu: "Hematopoietic stem cells in the mouse embryonic yolk sac." dans: Stem cells (Dayton, Ohio), Vol. 14, Issue 3, pp. 269-80, (1996) (PubMed).

    Morrison, Hemmati, Wandycz, Weissman: "The purification and characterization of fetal liver hematopoietic stem cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 22, pp. 10302-6, (1995) (PubMed).

    Morrison, Weissman: "The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype." dans: Immunity, Vol. 1, Issue 8, pp. 661-73, (1995) (PubMed).

    Spangrude, Brooks: "Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells." dans: Blood, Vol. 82, Issue 11, pp. 3327-32, (1994) (PubMed).

    Ivanov, Fleming, Malek: "Regulation of nuclear factor-kappa B and activator protein-1 activities after stimulation of T cells via glycosylphosphatidylinositol-anchored Ly-6A/E." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2394-406, (1994) (PubMed).

    Jurecic, Van, Belmont: "Enrichment and functional characterization of Sca-1+WGA+, Lin-WGA+, Lin-Sca-1+, and Lin-Sca-1+WGA+ bone marrow cells from mice with an Ly-6a haplotype." dans: Blood, Vol. 82, Issue 9, pp. 2673-83, (1993) (PubMed).

    Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." dans: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

    Flood, Dougherty, Ron: "Inhibition of Ly-6A antigen expression prevents T cell activation." dans: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 115-20, (1990) (PubMed).

    Codias, Malek: "Regulation of B lymphocyte responses to IL-4 and IFN-gamma by activation through Ly-6A/E molecules." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 6, pp. 2197-204, (1990) (PubMed).

    Codias, Rutter, Fleming, Malek: "Down-regulation of IL-2 production by activation of T cells through Ly-6A/E." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 5, pp. 1407-14, (1990) (PubMed).

    Spangrude, Klein, Heimfeld, Aihara, Weissman: "Two monoclonal antibodies identify thymic-repopulating cells in mouse bone marrow." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 2, pp. 425-30, (1989) (PubMed).

    van de Rijn, Heimfeld, Spangrude, Weissman: "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 12, pp. 4634-8, (1989) (PubMed).

    Spangrude, Aihara, Weissman, Klein: "The stem cell antigens Sca-1 and Sca-2 subdivide thymic and peripheral T lymphocytes into unique subsets." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 11, pp. 3697-707, (1988) (PubMed).

    Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." dans: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, (1988) (PubMed).

    Aihara, Bühring, Aihara, Klein: "An attempt to produce "pre-T" cell hybridomas and to identify their antigens." dans: European journal of immunology, Vol. 16, Issue 11, pp. 1391-9, (1987) (PubMed).

    Malek, Ortega, Chan, Kroczek, Shevach: "Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies." dans: The Journal of experimental medicine, Vol. 164, Issue 3, pp. 709-22, (1986) (PubMed).

  • Antigène
    Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
    Autre désignation
    Ly-6A/E (Ly6a Produits)
    Synonymes
    anticorps Ly-6A.2, anticorps Ly-6A/E, anticorps Ly-6E.1, anticorps Sca-1, anticorps Sca1, anticorps TAP, anticorps lymphocyte antigen 6 complex, locus A, anticorps Ly6a
    Sujet
    Synonyms: Sca-1
    Pathways
    Sensory Perception of Sound, Activated T Cell Proliferation
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