LY6D anticorps

N° du produit ABIN2689823

Cet anticorps Rat Monoclonal détecte spécifiquement LY6D dans FACS, CyTox, IHC (fro) et IP. Il présente une réactivité envers Souris et a été mentionné dans 6+ publications.

Aperçu rapide pour LY6D anticorps (ABIN2689823)

Antigène: LY6D (Lymphocyte Antigen 6 Complex, Locus D (LY6D))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LY6D est non-conjugé

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: 49

Détail du produit anti-LY6D anticorps

Marque: BD Pharmingen™

Attributs du produit: The 49-H4 antibody reacts with the ThB antigen (Ly-6D), which is a 15- kDa GPI-anchored protein of the Ly-6 multigene family. ThB has been detected on cortical thymocytes, small sIg- Thy-1- and sIg+ lymphoid cells in the bone marrow, thymic medullary epithelial cells, all epidermal layers (except the stratum corneum), and on peripheral B lymphocytes, but not on peripheral T lymphocytes. It has been noted that there is strain-to-strain variation in the expression of the ThB antigen on splenic B cells, those of strains with the Ly-6.2 haplotype (e.g., AKR, C57BL, DBA/2, SJL, SWR) stain more intensely with anti- ThB reagents than those of Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, NZB), and B cells of hybrids of Ly-6.1 and Ly-6.2 strains stain with intermediate intensity. The proportions of the ThB+ B cells and thymocytes do not differ significantly among strains.

BD Pharmingen™ Purified Rat Anti-Mouse Ly-6D - Purified - Clone 49-H4 - Isotype Rat IgG2c, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: BALB/c mouse plasmacytoma MOPC-104E

Isotype: IgG2c kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-LY6D (ABIN2689823)

Gumley, McKenzie, Sandrin: "Polymorphism at the mouse Thb locus." dans: Immunogenetics, Vol. 39, Issue 6, pp. 390-4, (1994) (PubMed).

Boyd, Tucek, Godfrey, Izon, Wilson, Davidson, Bean, Ladyman, Ritter, Hugo: "The thymic microenvironment." dans: Immunology today, Vol. 14, Issue 9, pp. 445-59, (1993) (PubMed).

Gumley, McKenzie, Kozak, Sandrin: "Isolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB)." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2615-8, (1992) (PubMed).

Godfrey, Izon, Tucek, Wilson, Boyd: "The phenotypic heterogeneity of mouse thymic stromal cells." dans: Immunology, Vol. 70, Issue 1, pp. 66-74, (1990) (PubMed).

Matossian-Rogers, Rogers, Ledbetter, Herzenberg: "Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6." dans: Immunogenetics, Vol. 15, Issue 6, pp. 591-9, (1982) (PubMed).

Eckhardt, Herzenberg: "Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression." dans: Immunogenetics, Vol. 11, Issue 3, pp. 275-91, (1981) (PubMed).

Détails sur LY6D

Antigène: LY6D (Lymphocyte Antigen 6 Complex, Locus D (LY6D))

Autre désignation: Ly-6D

Sujet: Synonyms: ThB