LY6C + LY6G anticorps (Biotin)

N° du produit ABIN2689825

Cité dans 6 publications. Cet anticorps anti-LY6C + LY6G Monoclonal Rat conjugué à Biotin (Clone RB6) (ABIN2689825) détecte spécifiquement LY6C + LY6G dans FACS et Sep. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour LY6C + LY6G anticorps (Biotin) (ABIN2689825)

Antigène: LY6C + LY6G

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS), Separation (Sep)

Clone: RB6

Détail du produit

Attributs du produit: The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the "lineage cocktail" used in studies of hematopoietic lineages. The mAb 1A8 (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse Ly-6G and Ly-6C - Biotin - Clone RB6-8C5 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Not Reported

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Référence

Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." dans: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).

Conlan, North: "Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody." dans: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 259-68, (1994) (PubMed).

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, (1993) (PubMed).

Tepper, Coffman, Leder: "An eosinophil-dependent mechanism for the antitumor effect of interleukin-4." dans: Science (New York, N.Y.), Vol. 257, Issue 5069, pp. 548-51, (1992) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).

Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." dans: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, (1989) (PubMed).

Détail du antigène

Antigène: LY6C + LY6G

Autre désignation: Ly-6G and Ly-6C

Sujet: Synonyms: Gr-1