NEUROD1 anticorps

N° du produit ABIN2689856

Détail du produit anti-NEUROD1 anticorps

Antigène: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEUROD1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Marque: BD Pharmingen™

Attributs du produit: The R8-294 monoclonal antibody specifically binds to Neurogenic differentiation factor 1 (NeuroD1). NeuroD1 is a basic helix-loop-helix (bHLH) transcription factor that is also known as BHLHA3, Beta-cell E-box transactivator 2, BETA2 and NeuroD. NeuroD1 interacts with other bHLH proteins and binds the insulin E-box sequence to regulate pancreatic islet cell development. It also plays a key role in central nervous system (CNS) and sensory nervous system neuron development. Specifically, it is required for the terminal differentiation of neurons during late stages of neurogenesis in the CNS (cerebral cortex, hippocampus, and cerebellum) and the sensory nervous system (eye, inner ear, and olfactory system). During antibody development, the purified R8-294 monoclonal antibody was found to detect NeuroD1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis. Western Blot analysis of NeuroD1 in human Retinoblastoma cell lysate. Lysate from Y79 cells (ATCC, HTB-18™) was probed with Purified Mouse Anti-NeuroD1 monoclonal antibody at titrations of 0.125 (lane 1), 0.06 (lane 2), and 0.03 μg/mL (lane 3). NeuroD1 is identified as a band of approximately 45 kDa. Immunoflourescent staining of NeuroD1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 Buffer, and stained with Purified Mouse Anti-NeuroD1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.

BD Pharmingen™ Purified Mouse Anti-NeuroD1 - Purified - Clone R8-294 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human NeuroD1 Recombinant Protein

Clone: R8

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-NEUROD1 (ABIN2689856)

Cherry, Wang, Bormuth, Schwab, Olson, Cepko: "NeuroD factors regulate cell fate and neurite stratification in the developing retina." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 20, pp. 7365-79, (2011) (PubMed).

Pang, Yang, Vierbuchen, Ostermeier, Fuentes, Yang, Citri, Sebastiano, Marro, Südhof, Wernig: "Induction of human neuronal cells by defined transcription factors." dans: Nature, Vol. 476, Issue 7359, pp. 220-3, (2011) (PubMed).

Gao, Ure, Ables, Lagace, Nave, Goebbels, Eisch, Hsieh: "Neurod1 is essential for the survival and maturation of adult-born neurons." dans: Nature neuroscience, Vol. 12, Issue 9, pp. 1090-2, (2009) (PubMed).

Kuwabara, Hsieh, Muotri, Yeo, Warashina, Lie, Moore, Nakashima, Asashima, Gage: "Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis." dans: Nature neuroscience, Vol. 12, Issue 9, pp. 1097-105, (2009) (PubMed).

Habener, Kemp, Thomas: "Minireview: transcriptional regulation in pancreatic development." dans: Endocrinology, Vol. 146, Issue 3, pp. 1025-34, (2005) (PubMed).

Cho, Tsai: "The role of BETA2/NeuroD1 in the development of the nervous system." dans: Molecular neurobiology, Vol. 30, Issue 1, pp. 35-47, (2004) (PubMed).

Tamimi, Steingrimsson, Copeland, Dyer-Montgomery, Lee, Hernandez, Jenkins, Tapscott: "The NEUROD gene maps to human chromosome 2q32 and mouse chromosome 2." dans: Genomics, Vol. 34, Issue 3, pp. 418-21, (1996) (PubMed).

Naya, Stellrecht, Tsai: "Tissue-specific regulation of the insulin gene by a novel basic helix-loop-helix transcription factor." dans: Genes & development, Vol. 9, Issue 8, pp. 1009-19, (1995) (PubMed).

Détails sur NEUROD1

Antigène: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Autre désignation: NeuroD1 (NEUROD1 Produits)

Synonymes: anticorps BETA2, anticorps BHF-1, anticorps MODY6, anticorps NEUROD, anticorps bHLHa3, anticorps Neurod, anticorps neurod, anticorps XNeuroD, anticorps MGC82572, anticorps NEUROD1, anticorps ND1, anticorps beta2, anticorps bhf-1, anticorps neuro-D, anticorps nrd, anticorps neuroD, anticorps neurod1, anticorps Beta2, anticorps neuronal differentiation 1, anticorps neurogenic differentiation 1, anticorps neuronal differentiation 1 S homeolog, anticorps neuronal differentiation 1 L homeolog, anticorps NEUROD1, anticorps Neurod1, anticorps neurod1.S, anticorps neurod1, anticorps neurod1.L

Sujet: Synonyms: Class A basic helix-loop-helix protein 3, bHLHa3, BETA2, NeuroD

Poids moléculaire: ~45 kDa

Pathways: Dopaminergic Neurogenesis, Hormone Transport, Carbohydrate Homeostasis