Cité dans 3 publications.
Cet anticorps anti-Ly76 Monoclonal Rat conjugué à Biotin (Clone TER-119) (ABIN2689924) détecte spécifiquement Ly76 dans FACS et IHC.
L’anticorps est réactif avec des échantillons de Souris.
Aperçu rapide pour Ly76 anticorps (Biotin) (ABIN2689924)
Antigène
Ly76
(Lymphocyte Antigen 76 (Ly76))
Reactivité
Souris
Hôte
Rat
Clonalité
Monoclonal
Conjugué
Cet anticorp Ly76 est conjugé à/à la Biotin
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC)
Clone
TER-119
Attributs du produit
The TER-119 antibody reacts with a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the "lineage cocktail" used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse TER-119/Erythroid Cells - Biotin - Clone TER-119 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Kina, Ikuta, Takayama, Wada, Majumdar, Weissman, Katsura: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." dans: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, (2000) (PubMed).
Kitajima, Kojima, Nakajima, Kondo, Hara, Miyajima, Takeuchi: "Definitive but not primitive hematopoiesis is impaired in jumonji mutant mice." dans: Blood, Vol. 93, Issue 1, pp. 87-95, (1999) (PubMed).
Ikuta, Kina, MacNeil, Uchida, Peault, Chien, Weissman: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." dans: Cell, Vol. 62, Issue 5, pp. 863-74, (1990) (PubMed).