T Cell Receptor (TCR) V beta 12 anticorps (Biotin)

N° du produit ABIN2689952

Cet anticorps Souris Monoclonal détecte spécifiquement T Cell Receptor (TCR) V beta 12 dans FACS. Il présente une réactivité avec des échantillons de Souris. Il a été cité dans 3+ publications.

Aperçu rapide pour T Cell Receptor (TCR) V beta 12 anticorps (Biotin) (ABIN2689952)

Antigène: T Cell Receptor (TCR) V beta 12

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS)

Clone: MR11

Détail du produit

Attributs du produit: The MR11-1 antibody reacts with the Vβ 12 T-cell Receptor (TCR) of mice having the b haplotype (e.g., C57BL, C58, DBA/1) of the Tcrb gene complex1 The Tcrb-V12 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 12 TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-8 (Mlsf, Dvb11.1), Mtv-9 (Etc-1, Mlsf, Dvb11.2) and/or Mtv-11 (Mlsf, Dvb11.3) proviruses (eg, A, AKR, BALB/c, CBA/J, C3H, DBA/2). Activation of Vβ 12 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. C57BL/6 spleen T cells expressing Vβ 12 TCR are among the predominant responders to MAIDS virus superantigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vß12 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated MR11-1, PE-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553048/553049), and PE-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC, Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Mouse Vβ 12 T-Cell Receptor - Biotin - Clone MR11-1 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Not Reported

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Référence

Vacchio, Hodes: "Selective decreases in T cell receptor V beta expression. Decreased expression of specific V beta families is associated with expression of multiple MHC and non-MHC gene products." dans: The Journal of experimental medicine, Vol. 170, Issue 4, pp. 1335-46, (1989) (PubMed).

Haqqi, Banerjee, Anderson, David: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." dans: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).

Behlke, Chou, Huppi, Loh: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).

Détail du antigène

Antigène: T Cell Receptor (TCR) V beta 12

Autre désignation: Vbeta 12 T-Cell Receptor