T Cell Receptor (TCR) V beta 14 anticorps (Biotin)

N° du produit ABIN2689953

Il existe 6+ publications pour ce produit. L’anticorps anti-T Cell Receptor (TCR) V beta 14 Monoclonal Rat est utilisé pour la détection de T Cell Receptor (TCR) V beta 14 dans des échantillons de Souris. Il a été validé pour FACS.

Aperçu rapide pour T Cell Receptor (TCR) V beta 14 anticorps (Biotin) (ABIN2689953)

Antigène: T Cell Receptor (TCR) V beta 14

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS)

Clone: 14-2

Détail du produit

Attributs du produit: The 14-2 antibody reacts with the Vβ 14 T-Cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) halpotypes of the Tcrb gene complex. Vβ 14 TCR-expressing T lymphocytes are completely eliminated in mice expressing I-E and the superantigens encoded by Mtv-2 endogenous provirus and/or MMTV-C3H, MMTV-GR, or MMTV-D2.GD exogenous virus. Recognition of these determinants by Vβ 14 TCR-expressing T cells is dependent upon presentation by I-E. Plate bound 14-2 antibody activates Vβ 14 TCR-bearing T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vβ 14 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated 14-2 and PE-conjugated 145-2C11 (anti-CD3e, Cat. No. 553063/553064) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ .

BD Pharmingen™ Biotin Rat Anti-Mouse Vβ 14 T-Cell Receptor - Biotin - Clone 14-2 - Isotype Rat IgM, κ - Reactivity Ms - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse B10.A Helper T-Cell

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Référence

Tomonari, Fairchild, Rosenwasser: "Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection." dans: Immunological reviews, Vol. 131, pp. 131-68, (1993) (PubMed).

Golovkina, Chervonsky, Dudley, Ross: "Transgenic mouse mammary tumor virus superantigen expression prevents viral infection." dans: Cell, Vol. 69, Issue 4, pp. 637-45, (1992) (PubMed).

Acha-Orbea, Shakhov, Scarpellino, Kolb, Müller, Vessaz-Shaw, Fuchs, Blöchlinger, Rollini, Billotte: "Clonal deletion of V beta 14-bearing T cells in mice transgenic for mammary tumour virus." dans: Nature, Vol. 350, Issue 6315, pp. 207-11, (1991) (PubMed).

Choi, Kappler, Marrack: "A superantigen encoded in the open reading frame of the 3' long terminal repeat of mouse mammary tumour virus." dans: Nature, Vol. 350, Issue 6315, pp. 203-7, (1991) (PubMed).

Marrack, Kushnir, Kappler: "A maternally inherited superantigen encoded by a mammary tumour virus." dans: Nature, Vol. 349, Issue 6309, pp. 524-6, (1991) (PubMed).

Liao, Maltzman, Raulet: "Positive selection determines T cell receptor V beta 14 gene usage by CD8+ T cells." dans: The Journal of experimental medicine, Vol. 170, Issue 1, pp. 135-43, (1989) (PubMed).

Détail du antigène

Antigène: T Cell Receptor (TCR) V beta 14

Autre désignation: Vbeta 14 T-Cell Receptor