Cet anticorp TCR V beta 4 est conjugé à/à la Biotin
Application
Flow Cytometry (FACS)
Marque
BD Pharmingen™
Attributs du produit
The KT4 antibody reacts with the Vβ4 T-cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells. Two-color analysis of the expression of Vb 4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with biotinylated KT4 (Panel B), PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. no. 554057, both panels). Flow cytometry was performed on a FACScan™ .
BD Pharmingen™ Biotin Rat Anti-Mouse Vβ4 T-Cell Receptor - Biotin - Clone KT4 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.25 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène
KT4 B10.D2 mouse T-cell clone I3
Clone
KT4
Isotype
IgG2b kappa
Indications d'application
Optimal working dilution should be determined by the investigator.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Katz, Benoist, Mathis: "T helper cell subsets in insulin-dependent diabetes." dans: Science (New York, N.Y.), Vol. 268, Issue 5214, pp. 1185-8, (1995) (PubMed).
Sugihara, Fujiwara, Shearer: "Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 683-94, (1993) (PubMed).
Tomonari, Lovering, Spencer: "Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype." dans: Immunogenetics, Vol. 31, Issue 5-6, pp. 333-9, (1990) (PubMed).