CD16 anticorps

N° du produit ABIN7304776

L’anticorps anti-CD16 Monoclonal Souris est utilisé pour la détection de CD16 dans des échantillons de Humain. Il a été validé pour FACS, IF et WB.

Aperçu rapide pour CD16 anticorps (ABIN7304776)

Antigène: CD16

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: CB16

Détail du produit anti-CD16 anticorps

Fonction: Mouse monoclonal antibody to CD16

Specificité: Recognizes human CD16

Attributs du produit: Mouse monoclonal antibody to CD16

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD16.

Isotype: IgG1

Information d'application

Indications d'application: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet: CD16B, FCG3, FCGR3, IGFR3, Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD antigen CD16b

ID gène: 2215

UniProt: O75015

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process