CD235a/GYPA anticorps

N° du produit ABIN7687635

Cet anticorps anti-CD235a/GYPA Monoclonal Souris (Clone HIR2) (ABIN7687635) détecte spécifiquement CD235a/GYPA dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD235a/GYPA anticorps (ABIN7687635)

Antigène: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD235a/GYPA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HIR2

Détail du produit anti-CD235a/GYPA anticorps

Fonction: Mouse monoclonal antibody to CD235

Specificité: Recognizes human CD235

Attributs du produit: Mouse monoclonal antibody to CD235a

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD235a.

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CD235a/GYPA

Antigène: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Autre désignation: CD235a

Sujet: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD antigen CD235a

ID gène: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location