CD45RA anticorps

N° du produit ABIN7304834

Cet anticorps Souris Monoclonal détecte spécifiquement CD45RA dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD45RA anticorps (ABIN7304834)

Antigène: CD45RA

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HI100

Détail du produit anti-CD45RA anticorps

Fonction: Mouse monoclonal antibody to CD45RA

Specificité: Recognizes human CD45RA

Attributs du produit: Mouse monoclonal antibody to CD45RA

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD45RA.

Isotype: IgG2b

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CD45RA

Antigène: CD45RA

Autre désignation: CD45RA

Sujet: CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD antigen CD45

ID gène: 5788

UniProt: P08575