CD58 anticorps

N° du produit ABIN7304854

L’anticorps anti-CD58 Monoclonal Souris est utilisé pour la détection de CD58 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour CD58 anticorps (ABIN7304854)

Antigène: CD58

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD58 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: TS2-9

Détail du produit anti-CD58 anticorps

Fonction: Mouse monoclonal antibody to CD58

Specificité: Recognizes human CD58

Attributs du produit: Mouse monoclonal antibody to CD58

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD58.

Isotype: IgG1

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CD58

Antigène: CD58

Autre désignation: CD58

Sujet: LFA3, Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3, CD antigen CD58

ID gène: 965

UniProt: P19256