CD8 anticorps

N° du produit ABIN7687689

Cet anticorps Rat Monoclonal détecte spécifiquement CD8 dans FACS et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour CD8 anticorps (ABIN7687689)

Antigène: CD8

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 2-43

Détail du produit anti-CD8 anticorps

Fonction: Rat monoclonal antibody to CD8a

Specificité: Recognizes mouse CD8a

Attributs du produit: Rat monoclonal antibody to CD8

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified mouse CD8a

Isotype: IgG2b

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CD8

Antigène: CD8

Autre désignation: CD8

Sujet: MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD antigen CD8a, CD8B1, T-cell surface glycoprotein CD8 beta chain, CD antigen CD8b

ID gène: 12525