SKP2 anticorps

N° du produit ABIN271816

Cet anticorps Lapin Polyclonal détecte spécifiquement SKP2 dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour SKP2 anticorps (ABIN271816)

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SKP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SKP2 anticorps

Specificité: This antibody detects endogenous levels of SKP2/p45 protein. (region surrounding Pro399)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur SKP2

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Autre désignation: SKP2 / FBXL1

Sujet: The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase 2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F, and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.Synonyms: Cyclin-A/CDK2-associated protein p45, F-box/LRR-repeat protein 1, S-phase kinase-associated protein 2

Poids moléculaire: approx. 47 kDa

ID gène: 6502

NCBI Accession: NP_005974

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases