POLR2A/RPB1 anticorps
Aperçu rapide pour POLR2A/RPB1 anticorps (ABIN271906)
Antigène
Voir toutes POLR2A/RPB1 (POLR2A) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects endogenous levels of POLR2A protein. (region surrounding Pro1613)
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
Purification
- Affinity Chromatography using epitope-specific immunogen.
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Indications d'application
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ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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Autre désignation
- POLR2A
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Sujet
- RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.Synonyms: DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit RPB1, POLR2, POLRA, RNA polymerase II, RNA polymerase II 220 kd, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB220, RPOL2
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Poids moléculaire
- 217,240 kDa
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ID gène
- 5430
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NCBI Accession
- NP_000928
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UniProt
- P24928
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Pathways
- Regulatory RNA Pathways
Antigène
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