TOP2B anticorps

N° du produit ABIN271965

Cet anticorps anti-TOP2B Polyclonal Lapin (ABIN271965) détecte spécifiquement TOP2B dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour TOP2B anticorps (ABIN271965)

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TOP2B anticorps

Specificité: This antibody detects endogenous levels of Topoisomerase II β protein. (region surrounding Ala32)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur TOP2B

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Autre désignation: TOP2B

Sujet: Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.Synonyms: DNA topoisomerase 2-beta, DNA topoisomerase II beta isozyme

Poids moléculaire: approx. 183 kDa

ID gène: 7155

NCBI Accession: NP_001059

UniProt: Q02880