Actin anticorps (pan)

N° du produit ABIN271986

Cet anticorps anti-Actin est un anticorps Lapin Polyclonal détectant Actin dans WB, IF et IHC (p). Adapté pour Humain, Rat et Souris.

Aperçu rapide pour Actin anticorps (pan) (ABIN271986)

Antigène: Actin

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Actin anticorps

Épitope: pan

Specificité: This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)

Réactivité croisée (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity-chromatography

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Actin

Antigène: Actin

Sujet: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".

Poids moléculaire: approx. 45 kDa