Actin anticorps (pan)

N° du produit ABIN271986

Détail du produit anti-Actin anticorps

Antigène: Actin

Épitope: pan

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificité: This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)

Réactivité croisée (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity-chromatography

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Actin

Antigène: Actin

Abstract: Actin Produits

Synonymes: anticorps Act57, anticorps Act57A, anticorps Act57b, anticorps Actin, anticorps BEST:RE38067, anticorps C, anticorps CG10067, anticorps CG30294, anticorps Dmel\\CG10067, anticorps M, anticorps T10, anticorps act 57A, anticorps act57A, anticorps act57B, anticorps act57b, anticorps actin, anticorps anon-EST:CL2c12, anticorps 143060_f_at, anticorps CG7478, anticorps D, anticorps Dmel\\CG7478, anticorps act 79B, anticorps act79B, anticorps mus79B, anticorps Act(88F), anticorps Act88-F, anticorps Act88f, anticorps CG5178, anticorps Dmel\\CG5178, anticorps E, anticorps Ifm(3)7, anticorps act 88F, anticorps act88F, anticorps rsd, anticorps Act87e, anticorps CG18290, anticorps Dmel\\CG18290, anticorps act 87E, anticorps act87E, anticorps mus87E, anticorps Act88F, anticorps Dsim\\GD19025, anticorps GD19025, anticorps dsim_GLEANR_2829, anticorps 42A, anticorps A, anticorps AFFX-Dros-ACTIN_M_r_at, anticorps Act, anticorps Act42, anticorps Act42a, anticorps Actin/BAP47, anticorps BAP47, anticorps CG12051, anticorps Dmel\\CG12051, anticorps act 42A, anticorps act42A, anticorps cytoplasmic, anticorps Actin 57B, anticorps Actin 79B, anticorps Actin 88F, anticorps Actin 87E, anticorps Actin-like protein C08B11.6, anticorps hypothetical protein, anticorps ACT_COLSC Actin (ISS), anticorps Actin 42A, anticorps actin gamma 1, anticorps actin, anticorps Actin, gamma 1, anticorps si:ch211-241j12.3, anticorps Act57B, anticorps Act79B, anticorps Act88F, anticorps Act87E, anticorps arp-6, anticorps Dsim\Act88F, anticorps ACTIN, anticorps Act42A, anticorps ACTG1, anticorps LOC548170, anticorps Bm1_16810, anticorps IDA5, anticorps LOC100342017, anticorps si:ch211-241j12.3

Sujet: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".

Poids moléculaire: approx. 45 kDa