NCF4 anticorps

N° du produit ABIN272048

L’anticorps anti-NCF4 Polyclonal Lapin est utilisé pour la détection de NCF4 dans des échantillons de Humain et Souris. Il a été validé pour IHC (p).

Aperçu rapide pour NCF4 anticorps (ABIN272048)

Antigène: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCF4 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NCF4 anticorps

Specificité: This antibody detects endogenous levels of p40 phox protein. (region surrounding Arg149)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur NCF4

Antigène: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Autre désignation: NCF-4

Sujet: Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation,including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase. However, p40-phox appears to downregulate oxidase function as well, by competing with an SH3 domain interaction between other essential oxidase components.Synonyms: Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4, SH3PXD4, p40-phox

Poids moléculaire: approx. 39 kDa

ID gène: 4689

NCBI Accession: NP_000622

UniProt: Q15080