PBK anticorps

N° du produit ABIN272056

Cet anticorps Lapin Polyclonal détecte spécifiquement PBK dans WB, IHC (p) et EIA. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour PBK anticorps (ABIN272056)

Antigène: PBK (PDZ Binding Kinase (PBK))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PBK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PBK anticorps

Specificité: This antibody detects endogenous levels of PBK/TOPK protein. (region surrounding Ile4)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 21000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should bedetermined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur PBK

Antigène: PBK (PDZ Binding Kinase (PBK))

Autre désignation: PBK / TOPK

Sujet: PBK (PDZ binding kinase) belongs to the serine/threonine protein kinase family. It is related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family and phosphorylates MAP kinase p38. It is active only in mitosis. When phosphorylated it forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. It makes a kinase-substrate complex with cdk1/cyclin B1 and PRC1 on microtubules during mitosis, enhances the cdk1/cyclin B1-dependent phosphorylation of PRC1 and thereby strongly promotes cytokinesis. It may play a role in the activation of lymphoid cells.Synonyms: CT84, Cancer/testis antigen 84, Lymphokine-activated killer T-cell-originated protein kinase, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, SPK, T-LAK cell-originated protein kinase

Poids moléculaire: approx. 36 kDa

ID gène: 55872

NCBI Accession: NP_060962

UniProt: Q96KB5