SP1 anticorps
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- Antigène Voir toutes SP1 Anticorps
- SP1 (Sp1 Transcription Factor (SP1))
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp SP1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificité
- This antibody detects endogenous levels of SP1 protein. (region surrounding Gly447)
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Pureté
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product SP1 Anticorps primaire
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- Indications d'application
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should bedetermined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- DO NOT FREEZE!
- Stock
- 4 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C.
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- Antigène
- SP1 (Sp1 Transcription Factor (SP1))
- Abstract
- SP1 Produits
- Synonymes
- anticorps fc18h09, anticorps zgc:85950, anticorps wu:fc18h09, anticorps SP1, anticorps 1110003E12Rik, anticorps AA450830, anticorps AI845540, anticorps Sp1-1, anticorps sp1 transcription factor, anticorps Sp1 transcription factor, anticorps Sp1 transcription factor L homeolog, anticorps trans-acting transcription factor 1, anticorps sp1, anticorps SP1, anticorps Sp1, anticorps sp1.L
- Sujet
- Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.Synonyms: Transcription factor Sp1
- Poids moléculaire
- approx. 90 kDa
- ID gène
- 6667
- NCBI Accession
- NP_003100
- UniProt
- P08047
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction
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