AIRE anticorps

N° du produit ABIN272137

Cet anticorps Lapin Polyclonal détecte spécifiquement AIRE dans WB et IF. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour AIRE anticorps (ABIN272137)

Antigène: AIRE (Autoimmune Regulator (AIRE))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AIRE est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-AIRE anticorps

Specificité: This antibody detects endogenous levels of AIRE-1 protein. (region surrounding Pro126)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur AIRE

Antigène: AIRE (Autoimmune Regulator (AIRE))

Autre désignation: AIRE / APECED

Sujet: The autoimmune regulator gene, which is defective in the hereditary autoimmune disease APECED, encodes the transcriptional activator AIRE. AIRE is expressed in the medullary epithelial cells and monocyte-dendritic cells of the thymus, with lower expression in the spleen, fetal liver and lymph nodes. In adult tissue, AIRE expression in the thymus is confined to the medulla and the cortico-medullary junction, where it is modulated by thymocytes undergoing negative selection. At the cellular level, AIRE is located in microtubular structures of the cytoskeleton and in discrete nuclear dots resembling ND10 nuclear bodies. AIRE is induced by developing early thymocytes and is associated with the correct establishment of a regular thymic environment. AIRE regulates thymic architecture via transcriptional control of downstream target genes. AIRE mutations in APECED patients may affect thymic T cell selection and the formation of self-tolerance.Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

Poids moléculaire: approx. 60 kDa

ID gène: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding