TOP3B anticorps

N° du produit ABIN272153

L’anticorps anti-TOP3B Polyclonal Lapin est utilisé pour la détection de TOP3B dans des échantillons de Humain et Souris. Il a été validé pour WB et IHC (p).

Aperçu rapide pour TOP3B anticorps (ABIN272153)

Antigène: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP3B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TOP3B anticorps

Specificité: This antibody detects endogenous levels of Topo IIIbeta-1 protein. (region surrounding Gln84)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur TOP3B

Antigène: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Autre désignation: TOP3B

Sujet: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I and DNA topoisomerase II relax both positive and negative supercoils, DNA topoisomerase III relaxes only negative supercoils. DNA topoisomerase III alpha exists as a long and a short isoform, which are produced by alternative splicing. DNA topoisomerase III alpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. DNA topoisomerase III beta exists as three isoforms, namely beta1, beta2 and beta3, also produced by alternative splicing. DNA topoisomerase III beta1 is expressed in testes, heart and skeletal muscle, whereas beta2 is expressed in thymus, kidney and pancreas.Synonyms: DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, TOP3B1

Poids moléculaire: approx. 96 kDa

ID gène: 8940

NCBI Accession: NP_003926

UniProt: O95985