UBTF anticorps
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- Antigène Voir toutes UBTF Anticorps
- UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp UBTF est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of UBF-1 protein. (region surrounding Glu536)
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Pureté
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product UBTF Anticorps primaire
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- Indications d'application
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- DO NOT FREEZE!
- Stock
- 4 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C.
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- Antigène
- UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))
- Autre désignation
- UBTF / UBF (UBTF Produits)
- Synonymes
- anticorps NOR-90, anticorps UBF, anticorps UBF-1, anticorps UBF1, anticorps A930005G04Rik, anticorps Tcfubf, anticorps wu:fj38c10, anticorps si:dz180g5.3, anticorps ubf, anticorps Ubtf, anticorps ubf1, anticorps xUBF, anticorps xUBFbeta2, anticorps UBTF, anticorps nor-90, anticorps ubf2, anticorps ubtf, anticorps upstream binding transcription factor, RNA polymerase I, anticorps upstream binding transcription factor, RNA polymerase I L homeolog, anticorps upstream binding transcription factor, RNA polymerase I S homeolog, anticorps UBTF, anticorps Ubtf, anticorps ubtf, anticorps ubtf.L, anticorps ubtf.S
- Sujet
- The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex. Two UBF proteins, of 97 kDa and 94 kDa, arise from the same gene as a result of alternative mRNA splicings. UBF activity is regulated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues. The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity. Expression of RNA may also be negatively regulated by the 70 kDa and 86 kDa Ku antigens.Synonyms: Autoantigen NOR-90, Nucleolar transcription factor 1, UBF-1, UBF1, Upstream-binding factor 1
- Poids moléculaire
- approx. 90 kDa
- ID gène
- 7343
- NCBI Accession
- NP_001070151
- UniProt
- P17480
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