CBX5 anticorps

N° du produit ABIN272193

Cet anticorps anti-CBX5 Polyclonal Lapin (ABIN272193) détecte spécifiquement CBX5 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour CBX5 anticorps (ABIN272193)

Antigène: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CBX5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CBX5 anticorps

Specificité: This antibody detects endogenous levels of HP1-alpha/CBX5 protein. (region surrounding Lys69)

Réactivité croisée (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1/10000-1/20000. Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CBX5

Antigène: CBX5 (Chromobox Homolog 5 (CBX5))

Autre désignation: CBX5

Sujet: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1), HP1α, HP1β and HP1γ. As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.Synonyms: Chromobox protein homolog 5, HP1 alpha, HP1-alpha, HP1A, Heterochromatin protein 1 homolog alpha, p25

Poids moléculaire: approx. 22 kDa

ID gène: 23468

NCBI Accession: NP_001120793

UniProt: P45973