CEBPE anticorps

N° du produit ABIN272198

L’anticorps anti-CEBPE Polyclonal Lapin est utilisé pour la détection de CEBPE dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour CEBPE anticorps (ABIN272198)

Antigène: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CEBPE est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CEBPE anticorps

Specificité: This antibody detects endogenous levels of C/EBP-epsilon protein. (region surrounding Ala217)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur CEBPE

Antigène: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Autre désignation: CEBPE

Sujet: The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.Synonyms: C/EBP epsilon, CCAAT/enhancer-binding protein epsilon

Poids moléculaire: approx. 28 kDa

ID gène: 1053

NCBI Accession: NP_001796

UniProt: Q15744

Pathways: Cellular Response to Molecule of Bacterial Origin