Actin anticorps (N-Term)

N° du produit ABIN2738124

L’anticorps Souris Monoclonal anti-Actin a été validé pour WB, IHC, ELISA, IF, IHC (p), IP et FACS. Il convient pour détecter Actin dans des échantillons de Humain, Rat, Souris, Poulet, Lapin, Chien, Boeuf (Vache), Singe, Porc, Cobaye, Cat, Babouin, Mouton et Chévre.

Aperçu rapide pour Actin anticorps (N-Term) (ABIN2738124)

Antigène: Actin

Reactivité: Humain, Rat, Souris, Poulet, Lapin, Chien, Boeuf (Vache), Singe, Porc, Cobaye, Cat, Babouin, Mouton, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 1A4

Détail du produit anti-Actin anticorps

Épitope: N-Term

Specificité: Human Actin

Purification: Protein G purified

Immunogène: An N-Terminal decapeptide of the alpha smooth muscle isoform.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle. however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin. No special pretreatment is required for the immunohistochemical staining of FFPE tissue. ELISA: Order BSA-free format.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.

Détails sur Actin

Antigène: Actin