BTLA anticorps (APC)
Aperçu rapide pour BTLA anticorps (APC) (ABIN2738632)
Antigène
Voir toutes BTLA AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Specificité
- Antibody from clone ANC6E9 binds to CD272 on the cell surface of human PBMC in Flow cytometry, and to recombinant CD272-muIg in EIA.
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Purification
- Protein A purified
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Immunogène
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Recombinant extracellular human CD272.
Type of Immunogen: Recombinant protein -
Isotype
- IgG1 kappa
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Indications d'application
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Approved: ELISA, Flo
Usage: Five x 105 human peripheral blood mononuclear cells (PBMC) per tube were washed and incubated 45 minutes on ice with 80 μL of anti-CD272/APC at a 1:50 dilution (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.89 log10 fluorescent units when compared to Mouse IgG1/APC Isotype Control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD272 antibody. ELISA: Use for capture. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Do not freeze.
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Stock
- 4 °C
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Stockage commentaire
- Store at 4°C. Do not freeze. Protect from light.
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- BTLA (B and T Lymphocyte Associated (BTLA))
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Autre désignation
- BTLA / CD272
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Sujet
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Name/Gene ID: BTLA
Synonyms: BTLA, BTLA1, B and T lymphocyte associated, CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, CD272 antigen -
ID gène
- 151888
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Pathways
- Cancer Immune Checkpoints
Antigène
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