CD8 anticorps (Cy7,PE)

N° du produit ABIN2739653

Cet anticorps Souris Monoclonal détecte spécifiquement CD8 dans FACS et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour CD8 anticorps (Cy7,PE) (ABIN2739653)

Antigène: CD8

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est conjugé à/à la Cy7,PE

Application: Flow Cytometry (FACS), ELISA

Clone: 14

Détail du produit anti-CD8 anticorps (Cy7,PE)

Specificité: Antibody 14 recognizes the CD8 alpha chain of approximately 33 kd (1).

Purification: Protein A purified

Immunogène: Mouse IgG1, kappa.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, Flo

Usage: Reagent was tested for binding to ficoll prepared human peripheral blood mononuclear cells (PBMC) in FACS. Five x 105 PBMC per tube were washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of antihuman CD8/PE-Cy7 at a 1:50 dilution (5 μg/mL). Cells were then washed three times, fixed and analyzed by FACS using a lymphoid gate. A 25.7 % sub population of the cells stained positive with a mean shift of 2.5 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-human CD8 antibody. ELISA: Use for detection. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 4°C. Do not freeze. Protect from light.

Détails sur CD8

Antigène: CD8

Autre désignation: CD8