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Cytokeratin 8/18 anticorps

L’anticorps Souris Monoclonal anti-Cytokeratin 8/18 a été validé pour WB, IF, FACS, IHC, IHC (p) et IP. Il convient pour détecter Cytokeratin 8/18 dans des échantillons de Humain.
N° du produit ABIN2740299

Aperçu rapide pour Cytokeratin 8/18 anticorps (ABIN2740299)

Antigène

Cytokeratin 8/18

Reactivité

  • 54
  • 11
  • 10
  • 10
  • 10
  • 8
Humain

Hôte

  • 46
  • 8
  • 1
Souris

Clonalité

  • 54
  • 1
Monoclonal

Conjugué

  • 13
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Cytokeratin 8/18 est non-conjugé

Application

  • 33
  • 32
  • 31
  • 23
  • 14
  • 12
  • 8
  • 3
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone

K8-8-DC10
  • Specificité

    Human Cytokeratin 8+18

    Purification

    Protein G purified

    Immunogène

    A keratin preparation from a human carcinoma (K8.8) and PMC-42 human breast carcinoma cells (DC10) were used as the immunogen for this Cytokeratin 8 + 18 antibody cocktail.

    Type of Immunogen: Cells

    Isotype

    IgG1 kappa
  • Indications d'application

    Approved: Flo (0.5 - 1 μg/10 x106), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

    Usage: Cytokeratins are a group of proteins expressed in pairs in keratinized and non-keratinized epithelial tissue. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). The pair are normally seen in simple but not stratified epithelial cells. This antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this antibody cocktail is indistinguishable from that obtained with monoclonal antibody 5D3.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C

    Stockage commentaire

    Store at 2°C to 8°C.
  • Antigène

    Cytokeratin 8/18

    Autre désignation

    Cytokeratin 8+18

    Sujet

    This antibody detects: Cytokeratin 8+18
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