EpCAM anticorps (Biotin)

N° du produit ABIN2740720

Cet anticorps anti-EpCAM est un anticorps Souris Monoclonal détectant EpCAM dans FACS et ELISA. Adapté pour Humain.

Aperçu rapide pour EpCAM anticorps (Biotin) (ABIN2740720)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: ANC8D4

Détail du produit anti-EpCAM anticorps (Biotin)

Specificité: Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.

Purification: Protein A purified

Immunogène: Recombinant human CD326(EpCAM).

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, Flo

Usage: Cultured human SW480 cells were harvested by EDTA. 5 x 10⁵ per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-human CD326/Biotin at concentration of 5 μg/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.93 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when cells were pre incubated with unlabeled antihuman CD326 mAb (#126-020). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 4°C, avoid freeze-thaw cycles.

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: ESA / EPCAM

Sujet: Name/Gene ID: EPCAM

Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1

ID gène: 4072