EpCAM anticorps (PE)

N° du produit ABIN2740721

Cet anticorps Souris Monoclonal détecte spécifiquement EpCAM dans FACS et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour EpCAM anticorps (PE) (ABIN2740721)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est conjugé à/à la PE

Application: Flow Cytometry (FACS), ELISA

Clone: ANC8D4

Détail du produit anti-EpCAM anticorps (PE)

Specificité: Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.

Purification: Protein A purified

Immunogène: Recombinant human CD326(EpCAM).

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, Flo

Usage: Five x 105 cultured human SW480 cells were harvested by trypsiniaztion, washed and pre incubated ~5 minutes with 20 μL of 250 μg/mL human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD326/R-PE at a dilution factor of 1:50 (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.77 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL antiCD326 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 4°C. Do not freeze. Protect from light.

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: ESA / EPCAM

Sujet: Name/Gene ID: EPCAM

Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1

ID gène: 4072